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. 2018 Aug 30;11(6):1307–1322. doi: 10.1016/j.tranon.2018.08.001

Figure 5.

Figure 5

ALDH1A1 expression silencing lowers cell proliferation, colony formation and sensitizes cells to chemoradiation treatment. (A) ALDH1A1 expression silencing in MiaPaCa-2 and Panc 05.04 cells was confirmed using western blot analysis. ALDH1A1 knockdown reduced cell proliferation (B), lowered cell viability/metabolic activity (C), and attenuated the number of colonies formed by these cell lines (D). Cell proliferation was determined using the BrdU incorporation assay while metabolic rate was quantified using the MTT assay. Data represents mean ± SD of three independent experiments. Statistical significance was determined using a two-sided t-test with P < .01 (**) and P < .001 (***) against shControl cells. (E) ALDH1A1 expression silencing sensitizes MiaPaCa-2 cells to gemcitabine, radiation and chemoradiation. (F) For Panc 05.04 cells, ALDH1A1 expression silencing sensitizes the cells to gemcitabine only with no effect observed upon radiation or chemoradiation. Cell death was assayed using annexin V/PI staining. Data represents mean ± SD of three independent experiments. Statistical significance was determined using a two-sided t-test with P < .05 (*), P < .01 (**), P < .001 (***), and P < .0001 (****) against shControl cells. (G) Bar chart representing the proliferation rate of the three cell lines used for in vitro experiments. MiaPaCa-2 has the fastest proliferation rate while Panc 05.04 has the slowest rate of proliferation.