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. 2018 Aug 30;11(6):1307–1322. doi: 10.1016/j.tranon.2018.08.001

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© 2018 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Analysis of cellular oxidative stress. (A) Quantification of absolute ROS levels at 2 hours and 24 hours post radiation showed no differences between shControl and shALDH1A1 in both cell lines. (B) DNA damage induced by radiation was assessed by quantifying absolute H2A.X phosphorylation (Ser139) levels with no significant differences observed in either cell line upon ALDH1A1 silencing. Analysis of glutathione metabolism intermediates revealed no changes in the levels of free oxidized thiol pool (C), free reduced thiol pool (D), oxidized plus protein-bound thiol pool (E), and the GSH/GSSG ratio (F) upon ALDH1A1 silencing in both cell lines.