Table 1.

Summary information on the number of callable sites, the total number of single base mutations, resulting mutation rate (μ) per generation and site, the number of single nucleotide mutations (SNMs) and the associated rate (μ SNM), number of alternative SNMs that occurred at the same site, number of insertions, deletions, transitions (Ts), and transversions (Tv) per mutation accumulation (MA) line

MA line	Number of callable sites	Number of mutations	μ	SNMs	μ(SNM)	Alternative SNM per site	Insertions	Deletions	Ts	Tv
A1	113,428,649	7	1.23 × 10–8	2	3.53 × 10–9	0	3	2	1	1
A2	126,279,134	4	6.34 × 10–9	2	3.17 × 10–9	1	1	1	1	0
A3	125,198,906	3	4.79 × 10–9	1	1.60 × 10–9	0	0	2	1	0
A4	119,583,116	7	1.17 × 10–8	4	6.69 × 10–9	0	2	1	2	2
A5	100,483,488	9	1.79 × 10–8	4	7.96 × 10–9	0	2	3	0	4
A6	129,246,425	4	6.19 × 10–9	2	3.09 × 10–9	0	0	2	0	2
A7	127,455,137	3	4.71 × 10–9	1	1.57 × 10–9	1	0	2	0	0
A8	128,191,107	7	1.09 × 10–8	4	6.24 × 10–9	0	0	3	2	2
A9	130,004,076	3	4.62 × 10–9	2	3.08 × 10–9	0	0	1	2	0
A10	129,317,412	4	6.19 × 10–9	4	6.19 × 10–9	0	0	0	4	0
SUM	1,229,187,450	51		26		2	8	17	13	11

We observed both TV and TS at sites with two alternative SNMs and these were therefore not listed to either of the two groups.