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Visualization of lesions on leaves of frostbite1 (fro1) or wild-type (WT) Arabidopsis ecotype C24 plants cultured on NH4+ and NO3− as the only nitrogen source. (A) Appearance of necrosis on leaf blades indicated by arrows and (B) necrosis stained with trypan blue. Representative leaf blades from three independent plant cultures are shown. Means with different letters are significantly different (p < 0.05) by ANOVA followed by Tukey’s test.
