Fig. 6.

Intervention with citrus flavonoids does not attenuate lesion size but slows aortic cholesterol accumulation. Ldlr^−/− mice were fed a HFHC diet for 12 weeks. Subsequently, the same mice were treated with flavonoids added to the HFHC diet for an additional 12 weeks. Aortae were dissected and the aortic sinus sectioned and analyzed histologically. A: Representative sections of the aortic sinus stained with oil red-O and hematoxylin and lesion area quantitation (n = 12–21 per group). Scale bar is 500 μm. B: Total cholesterol (TC), cholesteryl ester CE, and free cholesterol (FC) in whole aortae (n = 6–8 per group). C: Gene expression in whole aortae (n = 6–8 per group). Data represent the mean ± SEM. Different letters are statistically different by ANOVA with post hoc Tukey test (P < 0.05). Nar, naringenin; Nob, nobiletin; N.S., not significant.