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. 2018 Jul 9;59(9):1620–1629. doi: 10.1194/jlr.M084459

Fig. 2.

Fig. 2.

UPLC profile of an extract of human RPE [donor age 74, 1 eye (A–D)]. A: Chromatogram with monitoring at 430 nm absorbance (UPLC condition a). Top insets: UV-visible absorbance spectra of isoA2E, A2E, A2GPE, A2-DHP-PE, atRALdi-PE, and unknown A2PE species. The chromatogram represents analysis of six eyes. B: FLR monitoring at an excitation of 430 nm and emission of 600 nm. C, D: Selected ion chromatogram at m/z 970.7 and 998.7 with Rts corresponding to peaks 6 and 7 in A. E: UPLC chromatogram (430 nm monitoring) detecting a synthetic product derived from a mixture of 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (DPPE) incubated with all-trans-retinal. Insets: Mass spectrum (left) and UV-visible absorbance spectrum (right) corresponding to indicated peak (A2-DPPE m/z 1,222.9) (arrow).