FLR emission spectra of alkenyl ether lysoA2PE(P-18:0/0:0). A: Emission spectra of alkenyl ether lysoA2PE (100 μM) in DOPC (1 mM), 2% DMSO in PBS, SDS (1 mM), trialkyl-methylammonium chloride (TMAC; 1 mM), and 100% DMSO recorded from a 384-well microplate. FLR (relative FLR units, RFU) was recorded at an excitation of 440 nm. Emission maximum (nanometers) of each spectral profile is indicated. B: FLR emission spectra of alkenyl ether lysoA2PE in chloroform, 2% DMSO in PBS, and DMSO, recorded from a quartz cuvette.