Fig. 4.

OXLAMs induce liver cell death and lead to increased mitochondrial ASK1 and Caspase-3 activation. A: TUNEL staining of mouse liver sections (5 um). TUNEL-positive cells are highlighted by arrows and depicted as fold increase of TUNEL-positive cells (10× magnification, scale bar: 250 um). B: Immunoblot analysis of ASK1 in liver protein lysate and normalized to GAPDH. C: Immunoblot analysis of mitochondrial fractions and detection of P-ASK1, ASK1, and TXNIP. Porin was used as a loading control. Densitometric analysis was performed on background-subtracted blots and normalized to the low LA group. Data are expressed as mean ± standard error and are referred to low LA group, which was set at 1. *P < 0.05, **P < 0.01; n.s., not significant (one-way ANOVA with Bonferroni post hoc test).