Fig. 5.

Effects of NT-PGC-1α deficiency on EE and RER during β₃-AR stimulation. A: Body weight and body composition of 7 week old WT and NT-PGC-1α^−/− mice housed at 28°C for 3 weeks (n = 8 per genotype). B: Acute thermogenic response of WT and NT-PGC-1α^−/− mice to an β₃-AR agonist CL316243. Mice (n = 7 or 8 per genotype) were measured for VO₂ and VCO₂ prior to and after 4 h stimulation with CL316243 at 30°C. EE was calculated as described in Materials and Methods and expressed per body weight (BW). Data are presented as mean ± SEM. ^#P < 0.05 determined by two-way ANOVA. C: Effect of prolonged β₃-AR stimulation on EE in WT and NT-PGC-1α^−/− mice at 30°C (n = 8 per genotype). D: Food intake of WT and NT-PGC-1α^−/− mice during 6 days of indirect calorimetry (n = 8 per genotype). E: Effect of prolonged β₃-AR stimulation on RER (VCO₂/VO₂) in WT and NT-PGC-1α^−/− mice (n = 8 per genotype). All data are presented as mean ± SEM. *P < 0.05; **P < 0.01; ^#P < 0.0000001 determined by Student’s t-test.