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Under TEM, the number of autophagosomes per 1 μm2 cytoplasmic area were 0.17 ± 0.06 (young fibroblasts) and 0.32 ± 0.21 (aged fibroblasts) (p = 0.154). The number of autolysosomes per 1 μm2 cytoplasmic area were 0.19 ± 0.06 (young fibroblasts) and 0.28 ± 0.10 (aged fibroblasts) (p = 0.138). n = 5 in each group, and the numbers were counted in at least 12 cells. Data are provided as mean ± SD. p values < 0.05 were considered statistically significant.
