Table 1.
Identification of the constituents from S. adstringens aqueous extract (SAAE) by LC-DAD-MS/MS.
| Peak | RT (min) | Compound | UV (nm) | MF | Negative Mode (m/z) | Positive Mode (m/z) | ||
|---|---|---|---|---|---|---|---|---|
| MS [M − H]− (*) | MS/MS | MS [M + H]+ (*) | MS/MS | |||||
| 1 | 1.2 | PDE-PDE (B type) | 275 | C30H26O14 | 609.1250 (2.5) | 423, 305, 177 | 611.1411 (2.6) | 287, 263, 179 |
| PDE-PDE-PDE (B type) | C45H38O21 | 913.1803 (3.3) | 423, 305, 261, 243, 177 | 915.1955 (2.5) | - | |||
| di-hexoside | C12H22O11 | 341.1093 (1.0) | - | 365.1051 (0.9) | - | |||
| 2 | 2.4 | Gallic acid st | 270 | C7H6O5 | 169.0140 (1.7) | - | 171.0291 (1.9) | - |
| 3 | 2.9 | PDE-PDE (B type) | 275 | C30H26O14 | 609.1280 (4.9) | 423, 305, 177, 165 | 611.1392 (0.6) | 425, 299, 287, 275, 263, 179 |
| 4 | 3.6 | PDE-PDE (B type) | 276 | C30H26O14 | 609.1254 (0.8) | 423, 305, 177, 165 | 611.1395 (1.6) | 425, 299, 287, 263, 179 |
| 5 | 4.2 | PDE-PDE (B type) | 275 | C30H26O14 | 609.1259 (1.6) | 423, 305, 177, 165 | 611.1400 (0.7) | 425, 299, 287, 275, 263, 245, 179 |
| 6 | 4.3 | Gallocatechin st | 275 | C15H14O7 | 305.0673 (2.0) | 179 | 307.0812 (3.2) | 163, 159 |
| 7 | 6.1 | PDE-PDE (B type) | 275 | C30H26O14 | 609.1245 (0.8) | - | 611.1422 (4.3) | - |
| 8 | 10.3 | Epigallocatechin st | 274 | C15H14O7 | 305.0673 (1.9) | 167 | 307.0823 (3.4) | 195, 177, 163, 159 |
| 9 | 13.5 | PRO-PDE (B type) | 280 | C30H26O13 | 593.1312 (1.9) | 305, 177 | 595.1446 (0.1) | 427 |
| 10 | 15.5 | C-hexosyl O-pentosyl 5,7-dihydroxychromone | 257, 285, 327 sh | C21H26O13 | 485.1312 (2.4) | 353, 335, 245, 233, 215, 205 | 487.1460 (2.9) | 355, 337, 319, 289, 259, 235, 205 |
| 11 | 17.3 | C-hexosyl O-pentosyl 5,7-dihydroxychromone | 257, 295, 325 sh | C21H26O13 | 485.1317 (3.3) | 365, 353, 335, 263, 245, 263, 233, 215, 205 | 487.1452 (1.0) | 319, 301, 283, 259, 235, 205 |
| 12 | 19.0 | PCY-PDE (B type) | 278 | C30H26O13 | 593.1308 (1.3) | 305 | 595.1453 (1.1) | 427, 307, 289 |
| 13 | 19.8 | PDE-PDE benzoate (B type) | 278 | C37H30O16 | 729.1461 (3.5) | 423, 305, 287, 261, 177 | 731.1611 (0.6) | 425, 407, 299, 287, 275, 263 |
| 14 | 23.5 | NI | 280 | C26H32O12 | 535.1840 (3.6) | - | 537.1981 (2.7) | - |
| 15 | 35.4 | NI | 290, 330 | C32H36O16 | 675.1946 (2.2) | 245, 233, 215, 207 | 677.2088 (1.8) | 235, 191, 163 |
| 16 | 36.2 | NI | 300 | C33H38O17 | 705.2068 (4.5) | 573, 467, 365, 335, 317, 245, 237, 233, 215 | 707.2193 (1.6) | 325, 221, 191 |
*: error in ppm; st: confirmed by authentic standard; sh: shoulder; MF: molecular formula; RT: retention time; NI: non identified; PDE: prodelphinidin, PRO: prorobinetidin; PCY: procyanidin.