Figure 5.
DC-induced proliferation of CD4 effector and regulatory T cells. Splenic DCs from irradiated and control mice as well as CD4+C25− effector and CD4+CD25+ regulatory T cells from nonirradiated mice were isolated by magnetic cell sorting. DCs were in vitro cocultured with either effector T cells or regulatory T cells and T cell proliferation was evaluated by 3H-thymidine incorporation. The figure shows the ratio of cell proliferation between regulatory and effector T cells cocultured with DCs from either control or irradiated mice. Data represent the average of 3 independent experiments with DCs isolated from 2–4 independent mice/experiment, with bars indicating mean ± SD. Significance was tested by Student’s t-test with * p < 0.005.