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Overview of FnCpf1-assisted multiplex genome editing and transcriptional repression in Streptomyces. (A) Design of FnCpf1-mediated genome editing plasmid. Two strong promoters, ermEp* and kasOp*, were used to drive the expression of FnCpf1 and crRNA, respectively. (B) Broad applications of FnCpf1-assisted genome editing and ddCpf1-based gene repression in Streptomyces. HR, homology arm; HDR, homology-directed repair; NHEJ, nonhomologous end joining; CRISPRi, CRISPR interference.
