TABLE 1.
Sipanmycin biosynthesis gene cluster in Streptomyces sp. CS149
| Protein | Proposed function | Protein encoded in Streptomyces sp. ML694-90F3, % identity/similarity | Proteins by Streptomyces sp. Tü 6075 accession no., % identity/similarity |
|---|---|---|---|
| SipR1 | Transcriptional regulator (LuxR family) | IdnR1, 54/67 | WP_075268275, 97/98 |
| SipS3 | Nucleoside-diphosphate sugar epimerase | IdnS3, 76/84 | WP_075263857, 99/99 |
| SipS2 | UDP-glucose 6-dehydrogenase | IdnS2, 72/80 | WP_075263856, 99/99 |
| SipS1 | N-Acetylglucosaminyl deacetylase | IdnS1, 65/78 | WP_075263855, 99/99 |
| SipP5 | Type I PKS | IdnP5, 64/74 | WP_075263854, 98/98 |
| SipIB3 | Crotonyl-CoA carboxylase/reductase | WP_075263853, 99/99 | |
| SipIB1 | 3-Oxoacyl-ACP synthase | WP_075263852, 99/99 | |
| SipIB2 | 3-Hydroxybutyryl-CoA dehydrogenase | WP_075268274, 99/98 | |
| SipS15 | Glycosyltransferase | IdnS15, 40/50 | WP_075263851, 96/97 |
| SipP4 | Type I PKS | IdnP4, 62/70 | WP_075263850, 97/97 |
| SipP3 | Type I PKS | IdnP3, 65/73 | WP_075263849, 95/96 |
| SipR2 | Transcriptional regulator (TetR family) | IdnR2, 58/73 | WP_075263848, 99/100 |
| SipT2 | ABC transporter | IdnT2, 73/83 | WP_075263847, 99/99 |
| SipT1 | ABC transporter | IdnT1, 68/83 | |
| SipB | Thioesterase | IdnB, 58/72 | WP_075263846, 98/98 |
| SipP2 | Type I PKS | IdnP2, 64/72 | WP_079181603, 70/75 |
| SipP1 | Type I PKS | IdnP1, 60/69 | WP_079181603, 89/90 |
| SipO1 | Cytochrome P450 | IdnO1, 82/89 | WP_075263845, 99/99 |
| SipL7 | AMP-dependent synthetase | IdnL7, 64/73 | WP_075263844, 94/95 |
| SipL6 | Acyl carrier protein | IdnL6, 58/70 | WP_075263843, 96/97 |
| SipL1 | AMP-dependent synthetase | IdnL1, 70/81 | WP_075263842, 98/98 |
| SipL2 | Malonyl-CoA-ACP transacylase | IdnL2, 69/77 | WP_079181433, 94/94 |
| SipL3 | Aminotransferase | IdnL3, 74/80 | WP_075268271, 98/98 |
| SipL4 | Lysine 2,3-aminomutase | IdnL4, 75/86 | WP_075263841, 99/99 |
| SipL5 | l-Proline amide hydrolase | IdnL5, 75/84 | WP_075268270, 99/99 |
| SipS4 | Glycosyltransferase | IdnS4, 77/83 | WP_079181602, 99/99 |
| SipS7 | Glucose-1-phosphate thymidylyltransferase | IdnS7, 63/77 | WP_075263839, 99/99 |
| SipS6 | dTDP-glucose 4,6-dehydratase | IdnS6, 66/74 | WP_075263838, 97/98 |
| SipS10 | NDP-hexose 2,3-dehydratase | IdnS10, 51/61 | WP_075263837, 98/98 |
| SipS8 | N-Methyltransferase | IdnS8, 58/71 | |
| SipS9 | Glycosyltransferase | IdnS9, 47/57 | WP_075263836, 98/98 |
| SipS5 | C-Methyltransferase | WP_075263835, 99/99 | |
| SipO2 | P450-derived glycosyltransferase activator | IdnO2, 47/57 | WP_075263834, 95/95 |
| SipS14 | Glycosyltransferase | IdnS14, 60/76 | WP_075263833, 99/99 |
| SipS11 | NDP-hexose-3-ketoreductase | IdnS11, 68/75 | WP_079181432, 96/98 |
| SipS13 | dTDP-4-dehydro-6-deoxyglucose aminotransferase | IdnS13, 74/83 | WP_075263832, 99/98 |