. 2018 Aug 31;84(18):e01462-18. doi: 10.1128/AEM.01462-18

TABLE 4.

Oligonucleotides used for gene replacement in Streptomyces sp. CS149

Name	Sequence (5′–3′)	Restriction site	Use
149dGT47.5F	TATGAATTCCGAACTCGTCCTGCTCGT	EcoRI	sipS15 replacement (5′ flanking region) (1,067 bp)
149dGT47.5R	TATAAGCTTACCAGGCTGTGCAGTCGT	HindIII	sipS15 replacement (5′ flanking region) (1,067 bp)
149dGT47.3F	TATGGATCCGGAACTGCGGGAGGAGAT	BamHI	sipS15 replacement (3′ flanking region) (1,069 bp)
149dGT47.3R	TATTCTAGACGCTTGATGACCTTCACCTG	XbaI	sipS15 replacement (3′ flanking region) (1,069 bp)
149dGT66.5F	TATGAATTCTGAGCTCGGACAGCACATAG	EcoRI	sipS4 replacement (5′ flanking region) (1,357 bp)
149dGT66.5R	TATAAGCTTCGGAAACGACATCAACATGA	HindIII	sipS4 replacement (5′ flanking region) (1,357 bp)
149dGT66.3F	TATCATATGCGATCTCGTCCCCGAACT	NdeI	sipS4 replacement (3′ flanking region) (1,124 bp)
149dGT66.3R	TATTCTAGAGATCTGGCTGGTGTCGATG	XbaI	sipS4 replacement (3′ flanking region) (1,124 bp)
149dGT71.5F	TATGAATTCCCACCACGTCAACGTCCAG	EcoRI	sipS9 replacement (5′ flanking region) (1,101 bp)
149dGT71.5R	TATAAGCTTGAGGACGGCACGGTCAGC	HindIII	sipS9 replacement (5′ flanking region) (1,101 bp)
149dGT71.3F	TATCATATGGAGGTCGTGCCGCTGCTG	NdeI	sipS9 replacement (3′ flanking region) (1,127 bp)
149dGT71.3R	TATTCTAGACGATCTGTCGCTCCTGTACG	XbaI	sipS9 replacement (3′ flanking region) (1,127 bp)
149dGT74.5F	TATGAATTCATGCGAGGGAATAAGTGACG	EcoRI	sipS14 replacement (5′ flanking region) (1,408 bp)
149dGT74.5R	TATAAGCTTGCGAAGACCGTGAACAGAAC	HindIII	sipS14 replacement (5′ flanking region) (1,408 bp)
149dGT74.3F	TATGGATCCGTGACCAAGGACAGCGAGAG	BamHI	sipS14 replacement (3′ flanking region) (1,118 bp)
149dGT74.3R	TATTCTAGACCTGTCTGATCCGTTCCAGT	XbaI	sipS14 replacement (3′ flanking region) (1,118 bp)
149-DORF70.5F	TATGAATTCGAACATCCCGATCGTCTCC	EcoRI	sipS8 replacement (5′ flanking region) (1,420 bp)
149-DORF70.5R	TATAAGCTTTCTGCTCATTCTCGATGACG	HindIII	sipS8 replacement (5′ flanking region) (1,420 bp)
149-DORF70.3F	TATCATATGGCGTCTGTGACCGGAGCA	NdeI	sipS8 replacement (3′ flanking region) (1,377 bp)
149-DORF70.3R	TATTCTAGAGTGATGCGCACCGAGGAC	XbaI	sipS8 replacement (3′ flanking region) (1,377 bp)
149-DORF72.5F	TATGAATTCTCCCAACCGGTTTCTCAA	EcoRI	sipS5 replacement (5′ flanking region) (1,527 bp)
149-DORF72.5R	TATAAGCTTCTCGGCATGCAGAAATGG	HindIII	sipS5 replacement (5′ flanking region) (1,527 bp)
149-DORF72.3F	TATCATATGAGGCTGTCCGAGGCGTAGT	NdeI	sipS5 replacement (3′ flanking region) (1,369 bp)
149-DORF72.3R	TATTCTAGAGCGATCAGGTCGTGAATTTG	XbaI	sipS5 replacement (3′ flanking region) (1,369 bp)
149dAT76.5F	TATGAATTCAGTTGCAGCAGGAGATCCAC	EcoRI	sipS13 replacement (5′ flanking region) (1,125 bp)
149dAT76.5R	TATAAGCTTCGAGGATGGCGAGTTCAG	HindIII	sipS13 replacement (5′ flanking region) (1,125 bp)
149dAT76.3F	TATGGATCCGGAGTGACGCTCGACCAG	BamHI	sipS13 replacement (3′ flanking region) (1,419 bp)
149dAT76.3R	TATTCTAGATTCAACTCGATGCACGACAC	XbaI	sipS13 replacement (3′ flanking region) (1,419 bp)
149dP450_58.5F	TATTCTAGATCGAAGTTCCGCATGCTCAT	XbaI	sipO1 replacement (5′ flanking region) (2,028 bp)
149dP450_58.5R	TATGGATCCCCTCCATGTCGAACGGGTAG	BamHI	sipO1 replacement (5′ flanking region) (2,028 bp)
149dP450_58.3F	TATAAGCTTATTCAGTGGAAGTTCGGCGT	HindII	sipO1 replacement (3′ flanking region) (4,050 bp)
149dP450_58.3R	TATAGATCTGGTATGGGGCGTGGTTTGTA	BglII	sipO1 replacement (3′ flanking region) (4,050 bp)
149dP450_73.5F	TATTCTAGAGTCCGGTTCCTTCCCTACG	XbaI	sipO2 replacement (5′ flanking region) (2,022 bp)
149dP450_73.5R	TATCATATGAGCCTCGGGTCGATCTGATA	NdeI	sipO2 replacement (5′ flanking region) (2,022 bp)
149dP450_73.3F	TATAAGCTTGCTGCTTGAGAAACCGGTTG	HindIII	sipO2 replacement (3′ flanking region) (2,203 bp)
149dP450_73.3R	TATAGATCTCGATGATCTCTCGACGCTGA	BglII	sipO2 replacement (3′ flanking region) (2,203 bp)