TABLE 5.
Oligonucleotides used for PCR confirmation of Streptomyces sp. CS149 mutants
| Name | Sequence (5′–3′) | Gene | Amplicon size (bp) in: |
|
|---|---|---|---|---|
| Wild-type strain | Mutant strain | |||
| comp149dGT47.5 | TACGCGGAGTTCAAGGAACC | sipS15 | 1,223 | 1,739 |
| comp149dGT47.3b | CCGTTCACGAGAGGTCTGTG | |||
| comp149dGT66.5 | CAGGGGTCGGACGGTATTTC | sipS4 | 1,297 | 2,004 |
| comp149dGT66.3 | AGCGGTTCCTCCTGGTAGAT | |||
| comp149dGT71.5 | TCTTCCGGCGTTGTACTGTC | sipS9 | 1,262 | 1,769 |
| comp149dGT71.3 | GAACATCCCGATCGTCTCCG | |||
| comp149dGT74.5 | GCTGCTTGAGAAACCGGTTG | sipS14 | 1,474 | 1,684 |
| comp149dGT74.3 | GGTCCGCCTTCTCCTTGTC | |||
| comp149dMT70.5 | GACAGTACAACGCCGGAAGA | sipS8 | 1,266 | 2,037 |
| comp149dMT70.3 | GTGCACCCCGGAGAACTAC | |||
| comp149dMT72.5 | TGGGTGCGTCACTTATTCCC | sipS5 | 1,655 | 1,922 |
| comp149dMT72.3 | CAGCTGATCCTCACGGACG | |||
| comp149dAT76.5 | TGACGGCACATCTGACCTTC | sipS13 | 1,983 | 2,410 |
| comp149dAT76.3 | CTGGAGTCGCTGAACTTCGT | |||
| comp149dP450_58.5 | CCGAAGCACTACCACCATCT | sipO1 | 1,738 | 2,141 |
| comp149dP450_58.3 | AGCAGTACGCCTCGGTGAT | |||
| comp149dP450_73.5 | ATGCGAGGGAATAAGTGACG | sipO2 | 1,408 | 1,691 |
| comp149dP450_73.3 | GCGAAGACCGTGAACAGAAC | |||
| ermE | TAGCTTGCGAGTGTCCGT | sipR1 | ||
| 149-LuxR.3 | TATGAATTCATCGGCTACGAAGTGCTCTG | |||