TABLE 6.
Oligonucleotides used for genetic complementation of Streptomyces sp. CS149 mutants
| Name | Sequence (5′–3′) | Restriction site | Gene expression (size [bp]) |
|---|---|---|---|
| 149eGT47.F | TATGGATCCCCGATGTACACCCGATTCCC | BamHI | sipS15 (1,060) |
| 149eGT47.R | TATGAATTCGAGGTCTGTGTCGGTCGTG | EcoRI | |
| 149eGT66.F | TATGGATCCCAGGGGTCGGACGGTATTTC | BamHI | sipS4 (1,511) |
| 149eGT66.R | TATGAATTCGAGGAAAACCAGGCGGACAT | EcoRI | |
| 149eGT71.F | TATTCTAGATCTTCCGGCGTTGTACTGTC | XbaI | sipS9 (1,138) |
| 149eGT71.R | TATGAATTCACGATGGTGTTCCCGCTG | EcoRI | |
| 149eGT74.F | TATGGATCCGCTGCTTGAGAAACCGGTTG | BamHI | sipS14 (1,469) |
| 149eGT74.R | TATGAATTCGCCTTCTCCTTGTCGCGG | EcoRI | |
| 149eMT70.F | TATGGATCCCTCACGCTTGGGATGATCGT | BamHI | sipS8 (750) |
| 149eMT70.R | TATGAATTCGTGCTCCGGTCACAGACG | EcoRI | |
| 149eMT72.F | TATTCTAGACTGGCGGGATTTCGTTTGGA | XbaI | sipS5 (1,272) |
| 149eMT72.R | TATGAATTCCTCGGACTACGCCTCGGA | EcoRI | |
| 149eAT76.F | TATGGATCCCCATGGGGCCTGAACTCG | BamHI | sipS13 (1,202) |
| 149eAT76.R | TATGAATTCCTTCCCGTCTCACGCCAG | EcoRI | |
| 149eP450_58.F | TATGGATCCCCACGATGAGCACGGAGAAG | BamHI | sipO1 (1,203) |
| 149eP450_58.R | TATGAATTCCCTACCAGGTGACACGCAG | EcoRI | |
| 149eP450_73.F | TATGGATCCGCCGGATCATGACATGACCA | BamHI | sipO2 (1,344) |
| 149eP450_73.R | TATGAATTCTCCCAACCGGTTTCTCAAGC | EcoRI |