TABLE 1.
Cell growth and mcl-PHA accumulation in the recombinant P. putida A514 strains
| Culturea | Strain | Relevant plasmid | Doubling timeb (h) | VAc utilization (mM) | CDWd (mg/liter) | PHA titer (mg/liter) | PHA content (wt %) |
|---|---|---|---|---|---|---|---|
| High N (1 g/liter) | A514 WT | None | 4.8 ± 0.2 | 14.9 ± 0.01 | 664.1 ± 3.6 | 11 ± 2.0 | 1.7 ± 0.3 |
| APvan | pPvan | 4.7 ± 0.3 | 14.8 ± 0.01 | 664.6 ± 2.2 | 12 ± 1.2 | 1.8 ± 0.2 | |
| AphaJ4C1 | pTJ4C1 | 4.6 ± 0.3 | 15.0 ± 0.02 | 668.0 ± 3.5 | 23 ± 1.0 | 3.4 ± 0.2 | |
| AalkKphaGC1 | pTphaGC1 | 4.5 ± 0.2 | 14.9 ± 0.01 | 667.5 ± 4.8 | 90 ± 2.1 | 13.5 ± 0.3 | |
| AR-PxylA | pTRPxylA | 4.9 ± 0.4 | 15.0 ± 0.01 | 694.7 ± 11.1 | 12 ± 0.6 | 1.7 ± 0.2 | |
| Axyl_phaJ4C1 | pTPxylAphaGC1 | 4.8 ± 0.1 | 15.0 ± 0.02 | 702.7 ± 19.4 | 26 ± 1.7 | 3.7 ± 0.3 | |
| Axyl_alkKphaGC1 | pTPxylAphaJ4C1 | 4.8 ± 0.2 | 15.0 ± 0.01 | 714.8 ± 12.9 | 246 ± 17.3 | 34.4 ± 0.3 | |
| Low N (65 mg/liter) | A514 WT | None | 3.8 ± 0.3 | 6.5 ± 0.03 | 133.8 ± 10.9 | 23 ± 1.0 | 17.3 ± 1.8 |
| APvan | pPvan | 3.7 ± 0.2 | 6.6 ± 0.06 | 135.5 ± 5.8 | 23 ± 3.8 | 17.1 ± 2.1 | |
| AphaJ4C1 | pTJ4C1 | 3.7 ± 0.2 | 7.4 ± 0.05 | 133.0 ± 7.8 | 43 ± 1.5 | 32.6 ± 1.2 | |
| AalkKphaGC1 | pTphaGC1 | 3.9 ± 0.1 | 7.6 ± 0.05 | 145.2 ± 7.3 | 62 ± 6.1 | 42.4 ± 2.2 | |
| AR_PxylA | pTRPxylA | 3.8 ± 0.2 | 7.5 ± 0.04 | 148.7 ± 6.1 | 24 ± 0.6 | 16.6 ± 1.1 | |
| Axyl_phaJ4C1 | pTPxylAphaGC1 | 3.7 ± 0.3 | 7.9 ± 0.04 | 141.3 ± 12.3 | 63 ± 2.3 | 44.5 ± 4.9 | |
| Axyl_alkKphaGC1 | pTPxylAphaJ4C1 | 3.5 ± 0.1 | 8.7 ± 0.04 | 175.3 ± 13.3 | 116 ± 4.5 | 66.3 ± 4.7 |
a
The strains were grown in 15 mM vanillic acid-M9 medium under either high-N (1 g/liter) or low-N (65 mg/liter) conditions.
b
The doubling time (DT) was calculated during the exponential phase, based on the formula DT = t · [log 2/(log Nt − log N0)].
c
VA, vanillic acid.
d
CDW, cell dry weight.