TABLE 3.
Plasmids and bacterial strains used in this study
| Strain or plasmid | Relevant characteristic(s) | Source and/or reference |
|---|---|---|
| Plasmids | ||
| pPROBE-TT | 30 | |
| pPvan | pPROBE-TT derivative, P. putida A514 promoter of vanAB (Pvan) | This study |
| pTJ4C1 | pPROBE-TT derivative, Pvan, phaJ4, and phaC1 | This study |
| pTphaGC1 | pPROBE-TT derivative, Pvan, phaG, alkK, and phaC1 | This study |
| pTPxylA | pPROBE-TT derivative, P. putida A514 promoter of xylA (PxylA) | This study |
| pTRPxylA | pPROBE-TT derivative, PxylA, and xylR | This study |
| pTPxylAphaGC1 | pPROBE-TT derivative, PxylA, phaG, alkK, and phaC1 | This study |
| pTPxylAphaJ4C1 | pPROBE-TT derivative, PxylA, phaJ4, and phaC1 | This study |
| Strains | ||
| P. putida A514 | Wild type | Dennis Gross lab (TAMU) (17) |
| AT | A514 carrying pPROBE-TT | This study |
| APvan | A514 carrying pPvan | This study |
| AphaJ4C1 | A514 carrying pTJ4C1 | This study |
| AalkKphaGC1 | A514 carrying pTphaGC1 | This study |
| APxylA | A514 carrying pTPxylA | This study |
| AR-PxylA | A514 carrying pTRPxylA | This study |
| Axyl_phaJ4C1 | A514 carrying pTPxylAphaJ4C1 | This study |
| Axyl_alkKphaGC1 | A514 carrying pTPxylAphaGC1 | This study |