TABLE 4.
GC compositional analysis of mcl-PHA produced by recombinant P. putida A514 strainsa
| Strain | Culture | PHA composition (mol%)b |
||||
|---|---|---|---|---|---|---|
| 3HHx (C6) | 3HO (C8) | 3HD (C10) | 3HDD (C12) | 3HTD (C14) | ||
| APvan | High N (1 g/liter) | 56.0 ± 4.8 | ND | 21.7 ± 1.2 | 22.3 ± 1.0 | ND |
| AphaJ4C1 | 53.6 ± 2.1 | ND | 25.7 ± 1.5 | 20.7 ± 1.7 | ND | |
| AalkKphaGC1 | 15.0 ± 1.0 | 20.0 ± 1.9 | 47.8 ± 3.1 | 14.9 ± 2.2 | 2.3 ± 0.6 | |
| AR-PxylA | 57.4 ± 5.0 | ND | 19.9 ± 0.6 | 22.7 ± 1.0 | ND | |
| Axyl_phaJ4C1 | 56.8 ± 1.0 | ND | 20.6 ± 0.8 | 22.6 ± 1.1 | ND | |
| Axyl_ alkKphaGC1 | 10.2 ± 0.8 | 30.9 ± 1.8 | 41.2 ± 1.5 | 15.2 ± 0.7 | 2.5 ± 0.1 | |
| APvan | Low N (65 mg/liter) | 44.2 ± 0.3 | 8.3 ± 1.0 | 20.2 ± 1.2 | 13.4 ± 2.0 | 13.9 ± 0.6 |
| AphaJ4C1 | 33.2 ± 0.5 | 11.9 ± 0.8 | 25.8 ± 2.3 | 12.1 ± 1.5 | 17.0 ± 1.0 | |
| AalkKphaGC1 | 17.0 ± 1.1 | 18.3 ± 1.0 | 34.1 ± 2.1 | 12.1 ± 2.9 | 18.5 ± 0.3 | |
| AR-PxylA | 45.4 ± 2.8 | 7.3 ± 0.2 | 16.6 ± 2.0 | 14.9 ± 0.7 | 15.8 ± 0.4 | |
| Axyl_phaJ4C1 | 32.5 ± 0.9 | 9.8 ± 0.9 | 24.7 ± 2.3 | 13.8 ± 1.2 | 19.2 ± 0.1 | |
| Axyl_ alkKphaGC1 | 16.0 ± 0.8 | 15.2 ± 2.6 | 32.8 ± 1.7 | 13.1 ± 0.4 | 22.9 ± 0.1 | |
a
Vanillic acid (15 mM) was used as the carbon source.
b
3HHx, 3-hydroxyhexanoate; 3HO, 3-hydroxyoctanoate; 3HD, 3-hydroxydecanoate; 3HDD, 3-hydroxydodecanoate; 3HTD, 3-hydroxytetradecanoate; ND, not detected.