TABLE 2.
Primers used in this study
| Primer name | Sequencea | Purpose |
|---|---|---|
| PGPRB-10249 | TGGCAGCAGCCAACTCAGCTT | Insert validation in pET28a |
| PGPRB-10250 | TATAGGCGCCAGCAACCGCA | Insert validation in pET28a |
| PGPRB-10255 | GCTCACTCATTAGGCACCC | Insert validation in pJB3Tc20 |
| PGPRB-10256 | GGTAACGCCAGGGTTTTC | Insert validation in pJB3Tc20 |
| PGPRB-10409 | AGCGGATAACAATTTCACACAGG | Insert validation in pMMB67EH |
| PGPRB-10410 | TCTGTATCAGGCTGAAAATCTTCTCTC | Insert validation in pMMB67EH |
| PGPRB-10434 | TGGCTACCATGGGTCCAATGGAATTCCCTCCAACTAC | Cloning of paeM4BL03 in pET28a |
| PGPRB-10436 | TGGCTACTCGAGCAACTTATCTCCAGTGCCTGAT | Cloning of paeM4BL03 in pET28a |
| PGPRB-10440 | TGGCTACTGCAGCTTGCAGGACGTAGAATGCATGAG | Cloning of pmiCBL03 in pJB3Tc20 |
| PGPRB-10441 | TGGCTAGAATTCCTATCTCTTGATAGGTGCTTTCTCATCAC | Cloning of pmiCBL03 in pJB3Tc20 |
| PGPRB-10448 | GCCGCTGGTATACAAGAAGGA | Presence of paeM4 locus in P. aeruginosa genomes |
| PGPRB-10449 | GAGGAGTTCACCGTCAACGT | Presence of paeM4 locus in P. aeruginosa genomes |
| PGPRB-10473 | TGGCTAGAATTCAGTCACTTGGGGAGAGTTCCAGCATGAC | Cloning of hxuC in pMMB67EH |
| PGPRB-10474 | TGGCTAAAGCTTCCAATACTTCATGCTCGGCGACAACCG | Cloning of hxuC in pMMB67EH |
a
Restriction sites incorporated in primers are shown in bold, as follows: AAGCTT, HindIII; CCATGG, NcoI; CTCGAG, XhoI; CTGCAG, PstI; GAATTC, EcoRI.