FIG 4.

Thiaminase I rescues of thiamine auxotrophs. (A) ThiA_Cb rescued the growth of the BT10432 thiamine auxotrophs. (B) ThiA_Cb addition did not substantially improve the growth of the E264 thiamine auxotrophs. The growth curves displayed are the average of three replicate cultures with standard error bars at each time point. In each case, growth (OD₆₀₀) at 24 h was compared and significant differences are designated by different superscripts next to the names in the key (P < 0.0001 for all pairwise comparisons in these experiments).