FIG 5.

Growth of B. thailandensis strains on thiamine and its precursors. In the absence of thiaminase I, thiamine auxotrophs grew better on thiamine precursors than thiamine. (A) The growth curve showing BT10432-thiC reached a significantly higher OD₆₀₀ at 24 h (P = 0.0001) with HMP in contrast to thiamine. (B) E264-thiC reached a significantly higher OD₆₀₀ (P < 0.0001) at 24 h when rescued with HMP in contrast to thiamine. (C) BT10432-thiCthiG reached a significantly higher OD₆₀₀ (P < 0.0001) when rescued with HMP+THZ in contrast to thiamine. (D) No significant difference was observed between growth on thiamine and HMP+THZ for E264-thiCthiG at 24 h. The growth curves displayed are the average of six replicate cultures for precursors and nine for thiamine additions, with standard error bars at each time point. In each case, growth at 24 h was compared, and the significant differences are designated by different superscripts next to the names in the key.