TABLE 2.
Strains and plasmids used in this study
| Strain or plasmid | Genotypea | Source or reference |
|---|---|---|
| Organism strains | ||
| E. coli | ||
| DH5α | Invitrogen | |
| BL21(DE3) | Overexpression strain | Invitrogen |
| B. thailandensis | ||
| E264 | Wild type | 67 |
| BT10432 | E264 thiA::ISlacZ-PrhaBo-Tp/FRT | 63 |
| BT10432-thiC | BT10432 ΔthiC::tetR | This study |
| BT10432-thiG | BT10432 ΔthiG::cat | This study |
| BT10432-thiCthiG | BT10432 ΔthiC::tetR ΔthiG::cat | This study |
| E264-thiC | E264 ΔthiC::tetR | This study |
| E264-thiG | E264 ΔthiG::cat | This study |
| E264-thiCthiG | E264 ΔthiC::tetR ΔthiG::cat | This study |
| S. cerevisiae | ||
| InvSc1 | MATa/MATα leu2/leu2 trp1-289/trp1-289 ura3-52/ura3-52 his3-Δ1/his3-Δ1 | 62 |
| Plasmids | ||
| pMQ87 | CEN6 ARSH4 aaC1 lacZa oriT oriColE1; Gmr | 62 |
| pMQ87 thiC | pMQ87 ΔthiC::tetR | This study |
| pMQ87 thiG | pMQ87 ΔthiG::cat | This study |
| pET-28 bcmE | Overexpression vector containing bcmE(thiACb) | 25 |
a
Gmr, gentamicin resistance.