TABLE 2.
Primers used to identify YT4
| Target gene | Primer name | Primer sequence (5′–3′)a | Fragment length (bp)b | Source or reference |
|---|---|---|---|---|
| 16S rRNA | 27F | AGAGTTTGATCCTGGCTCAG | 1,392 | 41 |
| 1401R | ACGGGCGGTGTGTAC | |||
| gyrB | PfgyrBF | TGCACGGYGTRGGYGT | 939 | This study |
| PfgyrBR | CMGCRGAGTCACCTTCCA | |||
| rpoD | PsEG30F | ATYGAAATCGCCAARCG | 763 | 43 |
| PfrpoD 804R | CCTCRCCGATCGACATG | This study | ||
| rpoB | LAPS5 | TGGCCGAGAACCAGTTCCGCGT | 1,230 | 44 |
| LAPS27 | CGGCTTCGTCCAGCTTGTTCAG |
a
For sequencing, the same primers were used, except that 341F (5′-CCTACGGGAGGCAGCAG-3′) (42) was also used for the 16S rRNA gene in addition to 27F and 1401R. M, A+C; R, A+G; Y, C+T; K, G+T.
b
Values for the Pseudomonas fluorescens type strain listed in Table S1.