Table 1.

List of primers used for evaluating of in vitro differentiations of human dental pulp tissues harvested from extracted wisdom teeth (hDPSCs) into mesenchymal lineages and cholinergic neurons.

Gene	Primer Sequences	Product Size (bp)	Annealing Temp (°C)	Accession No.
RUNX2	F: 5′-ATGTGTGTTTGTTTCAGCAG-3′	199	60	NM_001024630.3
	R: 5′-TCCCTAAAGTCACTCGGTAT-3′
ON	F: 5′-GTGCAGAGGAAACCGAAGAG-3′	202	60	J03040.1
	R: 5′-AAGTGGCAGGAAGAGTCGAA-3′
BMP2	F: 5′-TAGACCTGTATCGCAGGCAC-3′	149	60	NM_001200.2
	R: 5′-GGTTGTTTTCCCACTCGTTT-3′
PPARγ2	F: 5′-TGCTGTCATTATTCTCAGTGGA-3′	124	60	AB565476.1
	R: 5′-GAGGACTCAGGGTGGTTCAG-3′
FABP4	F: 5′-TGAGATTTCCTTCATACTGGG-3′	128	60	NM_001442.2
	R: 5′-TGGTTGATTTTCCATCCCAT-3′
LPL	F: 5′-AGACACAGCTGAGGACACTT-3′	137	60	NM_000237.2
	R: 5′-GCACCCAACTCTCATACATT-3′
SOX9	F: 5′-ATGGAGCAGCGAAATCAACG-3′	118	60	BC007951.2
	R: 5′-CAAAGTCCAAACAGGCAGAGAG-3′
AGGRECAN	F: 5′-GAATGGGAACCAGCCTATACC-3′	98	60	NM_001135.3
	R: 5′-TCTGTACTTTCCTCTGTTGCTG-3′
COLLAGEN II	F: 5′-GAGACCTGAAACTCTGCCACC-3′	165	55	NM_001844.4
	R: 5′-TGCTCCACCAGTTCTTCTTGG-3′
ChAT	F: 5′-AGCAGAAATGCAGCCCTGAT-3′	182	60	NM_001142933.1
	R: 5′-GTCAGTCACGGCTCTCACAA-3′
HB9	F: 5′-CAAGCTCAACAAGTACCTGT-3′	134	56	NM_001165255.1
	R: 5′-GCTCTTTGGCCTTTTTGCTG-3′
ISL1	F: 5′-TGGTCATTGCCTTGCCAAAC-3′	106	56	XM_011543380.2
	R: 5′-TCAAACCAATGCAGCTCCAC-3′
YWHAZ (reference)	F: 5′-CTTCACAAGCAGAGAGCAAAG-3′	102	55	NM_003406.3
	R: 5′-CGACAATCCCTTTCTTGTCATC-3′

Abbreviation: F, forward primer sequence; R, reverse primer sequence; RUNX2, runt-related transcription factor 2; ON, osteonectin; BMP2, bone morphogenetic protein 2; PPARγ2, peroxisome proliferator activated receptor- γ2; FABP4, fatty acid binding protein 4; LPL, lipoprotein lipase; SOX9, sex determining region Y-box 9; AGGRECAN, cartilage-specific proteoglycan core protein; COLLAGEN II, type II collagen; ChAT, choline acetyltransferase; HB9, homeobox HB9; ISL1, insulin gene enhancer protein ISL-1; YWHAZ, Tyrosine 3-monooxygenease/ tryptophan 5-monooxygenease activation protein, zeta polypeptide.