Table 2.
The primers of Q-PCR in present study.
| Primer | Sequence | Tm (°C) | Length (bp) | Accession Number | PCR Efficiency% |
|---|---|---|---|---|---|
| Q-ZOG1-F | CCACCTCAACCAACTCCTACAC | 58.6 | 97 | XM_018648918 | 107 |
| Q-ZOG1-R | CTTAACTTGGCGGTTGTGAGTG | 60.1 | |||
| Q-GA2OX1-F | GCAGATAACAGGCTTGGACACTT | 60.4 | 123 | XM_009356581.2 | 108 |
| Q-GA2OX1-R | GATTTCCGAGTACCGAGATTGAA | 60.3 | |||
| Q-CKX7-F | GAGATTTGTGGAGCGGAAGAC | 58.8 | 188 | XM_009336405.2 | 109 |
| Q-CKX7-R | CCAGTAGAAACTAATCAAGCCAATA | 57.7 | |||
| Q-CKX6-F | AAAATCTGCTTACGACCCCTTGG | 63.8 | 124 | XM_009369812.2 | 96 |
| Q-CKX6-R | ACATGCCTTTAGGGCCTCTTCTT | 62.8 | |||
| Q-ACS-F | TGTCTCCTCATACACCGATACCC | 60.5 | 171 | XM_009365119.2 | 102 |
| Q-ACS-R | GAAAGAAGGTATCCACCACTCAA | 57.9 | |||
| Q-ACO-F | TCCCAGTTGTTGACTTGAGCCT | 61.2 | 194 | NM_001302321.1 | 93 |
| Q-ACO-R | CATTTCCTTAAACCTTTGCTCCA | 60.7 | |||
| Q-NAC25-F | TTCTACCCTAATCCTGCACTTCT | 57.3 | 118 | XM_009381159.2 | 108 |
| Q-NAC25-R | CATCTTAAACCCACCATCCAAA | 59.3 | |||
| Q-WOX1-F | TGTTACTGGGAGGCGTTTAGATT | 60.2 | 119 | XM_009361806 | 105 |
| Q-WOX1-R | AATACAATGGCGCTTATACAAGTC | 58.1 | |||
| Q-actin-F | CCATCCAGGCTGTTCTCTC | 54.7 | 139 | JN684184 | 100 |
| Q-actin-R | GCAAGGTCCAGACGAAGG | 55.7 | |||
| Q-UBI-F | ACCCTCGCCGACTACAAC | 55.3 | 198 | XM_009368893.2 | 94 |
| Q-UBI-R | ACTCCTTCCGCAGCCTCT | 55.4 |