Table 1.

Primers used for RT-PCR and PCR procedures.

Sanger
First Round Forward (Protease):5′-TGAARGAITGYACTGARAGRCAGGCTAAT-3′ Reverse (Protease): 5′-AYCTIATYCCTGGTGTYTCATTRTT-3′ Forward (RT): 5′-TTTYAGRGARCTYAATAARAGAACTCA-3′ Reverse (RT): 5′-CCTCITTYTTGCATAYTTYCCTGTT-3′
Second Round Forward (Protease): 5′-YTCAGRCAGRCCRGARCCAACAGC-3′ Reverse (Protease): 5′-CTGGTGTYTCATTRTTKRTACTAGGT-3′ Forward (RT): 5′- TTYTGGGARGTYCARYTAGGRATACC-3′ Reverse (RT): 5′- GGYTCTTGRTAAATTTGRTATGTCCA-3′
NGS
PR-INNER_F 5′-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCTTTAACTTCCCTCAGGTCACTCT-3′ RT-1_R 5′-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGTCAATGGCCATTGTTTAACTTTTGG-3′ RT-1_F 5′-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCAAAAGTTAAACAATGGCCATTGAC-3′ PRNEWIN_R 5′-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGCTGGTGTYTCATTRTTKRTACTAGGT-3′ 5FP127_F 5′-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGATACTGCATTTACCATACCTAG-3′ 3F262_R 5′-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGTCCCACTAACTTCTGTATGTC-3′