Table 3.
Mutations detected by Next-Generation Sequencing. The threshold for data analysis was set at 5%. Protease and RT genes were analyzed and the table includes the gene and compartment with drug resistance mutations detected at the 5% threshold. NP indicates that high frequency mutations were not present in the samples analyzed. Letters in bold indicate drug resistance mutations that were also detected by Sanger sequencing. (-) indicates not applicable.
| Sample | Gene | Classification | Surveillance | WT | Position | Mutation | Frequency | Coverage |
|---|---|---|---|---|---|---|---|---|
| 267966 | ||||||||
| Plasma | RT | NRTI | Yes | M | 184 | V | 99.4 | 46,490 |
| RT | NNRTI | No | E | 138 | A | 99.29 | 55,090 | |
| PBMCs | RT | NRTI | Yes | M | 184 | V | 42.23 | 69,897 |
| RT | NNRTI | No | E | 138 | A | 42.29 | 69,891 | |
| RT | Other | No | V | 179 | I | 41.47 | 69,896 | |
| CD4+ memory T-cells | RT | NRTI | Yes | M | 184 | V | 99.12 | 54,457 |
| RT | NNRTI | No | E | 138 | A | 99.38 | 54,452 | |
| 268249 | ||||||||
| Plasma | PR/RT | - | - | WT | - | NP | - | - |
| PBMCs | PR/RT | - | - | WT | - | NP | - | - |
| CD4+ memory T-cells | PR | Other | No | L | 10 | V | 12.69 | 9382 |
| 268138 | ||||||||
| Plasma | PR | Other | No | A | 71 | V | 99.17 | 20,609 |
| PBMCs | PR | Other | No | A | 71 | V | 98.64 | 66,474 |
| CD4+ memory T-cells | PR | Other | No | A | 71 | V | 98.49 | 79,020 |
| 275163 | ||||||||
| Plasma | PR | Other | No | K | 20 | R | 23.13 | 49,598 |
| PBMCs | PR | Other | No | K | 20 | R | 97.03 | 46,084 |
| CD4+ memory T-cells | PR | Other | No | K | 20 | R | 96.9 | 33,222 |