Table 1.
Primers Used in Cloning, qRT-PCR, and Vector Constructions.
| Primer Name | Primer Sequence (5′–3′) | Description |
|---|---|---|
| DlMFT 3′GSP1 | GGATTCACTGGATGGTCGT | 3′-RACE |
| DlMFT 3′GSP2: | TCAACAGAAGGCACCATTAG | |
| DlMFT 5′GSPa | CCTAATGGTGCCTTCTGTTG | 5′-RACE |
| DlMFT 5′GSPb | GACCATCCAGTGAATCCATT | |
| DlMFT-F | TTTTGGTTATGGCGGCTTC | Splice verification, gDNA amplification |
| DlMFT-R | ATGCTCCAGACCCCATACTA | |
| DlMFT-SP1 | GCGAACCAGTGAATCCATT | Promoter cloning |
| DlMFT-SP2 | CATCAGAGTGACCAGTGAGAGT | |
| DlMFT-SP3 | TGATGTCACAGCCGTTGGT | |
| DlMFT-pro-F | GCGGATCCTTTGCCTTGTCCAACTTCAC | For construction of DlMFT pro:GUS. The added BamI (GGATCC) and NcoI (CCATGG) sites were underlined |
| DlMFT-pro-R | GCCCATGGCAAACAAAGAGAAGAGAGGA | |
| DlMFT-SL-F | GAAGATCTATGGCGGCTTCGGTGGATC | Subcellar localization of DlMFT. The added BglII (AGATCT) site was underlined |
| DlMFT-SL-R | GAAGATCTGCGGCGACGGTTGGCCTTCC | |
| DlMFT-q-F | AACGGCTGTGACATCAAGC | qRT-PCR |
| DlMFT-q-R | CGACCATCCAGTGAATCCA | |
| NbEF1a-F | AGAGGCCCTCAGACAAAC | Reference gene for qRT-PCR in N. benthamiana |
| NbEF1a-R | TAGGTCCAAAGGTCACAA | |
| GeneRacerTM 3′-Primer | GCTGTCAACGATACGCTACGTAACG | 3′-RACE |
| GeneRacerTM 3′-Nested Primer | CGCTACGTAACGGCATGACAGTG | |
| GeneRacerTM 5′-Primer | CGACTGGAGCACGAGGACACTGA | 5′-RACE |
| GeneRacerTM 5′-Nested Primer | GGACACTGACATGGACTGAAGGAGTA |