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. 2018 Jul 25;19(8):2171. doi: 10.3390/ijms19082171

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(A) Effect of rhBMP-2 on proliferation in the hAMSCs seeded on the nHAC/PLA. The proliferation of the hAMSCs with rhBMP-2 is significantly higher than the cells without rhBMP-2. (B) Effect of rhBMP-2 on ALP activity of hAMSCs seeded on nHAC/PLA. (C) Effect of rhBMP-2 on mineral formation in the hAMSCs seeded on the nHAC/PLA. (D–F) Effect of rhBMP-2 on OCN, phosphate, and calcium content of hAMSCs seeded on nHAC/PLA. (D,E) The OCN and phosphate content in the experimental group of hAMSCs on nHAC/PLA were remarkedly higher compared with the control and blank control groups of hAMSCs seeded on nHAC/PLA during the 21 days of culture. (F) The calcium content in the experimental group of hAMSCs on nHAC/PLA was significantly lower than the control and blank control groups of hAMSCs on nHAC/PLA during the 21 days of culture. ( $\bar{x}$ ± s, n = 3, * p < 0.05, ** p < 0.01).