Table III.

Effect of BN combined with VCM on S aureus biofilms analyzed using crystal violet staining.

	OD570
Group	6 h	12 h	24 h	48 h
Blank	1.224±0.246	1.291±0253	1.226±0.194	1.283±0.272
1/2 MIC VCM	1.197±0.262	1.269±0.182	1.168±0.234	1.156±0.154
1 MIC VCM	1.211±0.287	1.264±0.267	1.151±0.163	1.112±0.116
2 MIC VCM	1.140±0.296	1.224±0.311	1.105±0.121	1.041±0.136a
1/2 MIC BN	1.211±0.291	1.021±0.180	0.961±0.067a	0.882±0.121a
1 MIC BN	1.112±0.287	1.048±0.196	0.904±0.127a,c	0.810±0.134a,c
2 MIC BN	0.965±0.134a	0.965±0.188a	0.877±0.176a,c	0.711±0.201a,c
1/2 MIC VCM+1/2 MIC BN	0.796±0.060a	0.916±0.160a	0.854±0.121a,c	0.747±0.146a,c
1/2 MIC VCM+1 MIC BN	0.945±0.149a,c	0.880±0.075a,c	0.802±0.876a,c	0.678±0.173a,c
1/2 MIC VCM+2 MIC BN	0.903±0.159a,c	0.857±0.160a,c	0.772±0.169a,c	0.644±0.170a,c
1 MIC VCM+1/2 MIC BN	0.882±0.148a,c	0.833±0.211a,c	0.757±0.120a,c	0.584±0.214a–c
1 MIC VCM+1 MIC BN	0.863±0.219a–c	0.805±0.177a–c	0.720±0.170a–c	0.526±0.222a–c
1 MIC VCM+2 MIC BN	0.833±0.199a–c	0.816±0.070a–c	0.654±0.182a–c	0.498±0.144a–c
2 MIC VCM+1/2 MIC BN	0.831±0.150a–c	0.776±0.093a–c	0.597±0.138a–c	0.469±0.158a–c
2 MIC VCM+1 MIC BN	0.768±0.149a–c	0.732±0.157a–c	0.553±0.223a–c	0.404±0.171a–c
2 MIC VCM+2 MIC BN	0.756±0.112a–c	0.645±0.116a–c	0.507±0.209a–c	0.390±0.160a–c

^aP<0.05 vs. control

^bP<0.05 vs. 1/2 and 1 MIC BN

^cP<0.05 vs. VCM. BN, brazilin; VCM, vancomycin; MIC, minimum inhibitory concentration; OD, optical density.