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. 2018 Aug 28;8:343. doi: 10.3389/fonc.2018.00343

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Copyright © 2018 Bamps, Dok and Nuyts.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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LLLT induces proliferation via increase of Ki67 levels and mitotic figures in HNSCC SQD9 cell line. (A) The percentage of Ki67 staining in SQD9 cells after irradiation with energy densities of 0, 1 and 2 J/cm2. (B) The number of mitotic figures present in SQD9 cells after irradiation with energy densities of 0, 1, and 2 J/cm2. (C) Ki67 and mitotic figure staining in SQD9 cells after irradiation with an energy density of 0 J/cm2. (D) Ki67 and mitotic figure staining in SQD9 cells after irradiation with an energy density of 1 J/cm2. (E) Ki67 and mitotic figure staining in SQD9 cells after irradiation with an energy density of 2 J/cm2. (A,B) Scoring of Ki67 staining (RM-9106-R7, Thermo Scientific) and mitotic figures was performed in 10 fields with a magnification of 200X by 2 independent observers. ^*p-values < 0.05 were calculated by a two-tailed t-test. Scale bar = 100 μm.