Figure 2.

EZR-AS1 knockdown suppresses BC cell proliferation and induces apoptosis. (A) Reverse transcription-quantitative polymerase chain reaction was performed to assess EZR-AS1 expression in MCF7 and MDA-MB-231 cells transfected with sh-NC or sh-EZR-AS1. Cell Counting Kit 8 assays were performed to assess the proliferation of (B) MCF7 and (C) MDA-MB-231 cells. (D) The percentage of (D) cells in S phase and (E) apoptotic MCF7 and MDA-MB-231 cells was assessed using flow cytometry. *P<0.05 vs. siNC. All data are presented as the mean ± standard deviation of three independent experiments. BC, breast cancer; sh, short hairpin RNA; NC, negative control.