Fig. 3.

Hypermethylation in promoter and decreased expression of GSTP1 and TXNRD2 in HMC. (A-B) Diagram of the positions of tested CpG units within the promoters of GSTP1 and TXNRD2. (C) Sequenom MassARRAY experiments using expanded sample size confirmed that one CpG unit in GSTP1 and one in the TXNRD2 CpG island promoter were statistically hypermethylated in the HMC group compared to the ARC group. n = 12 individual patients. (D) Relative mRNA levels of GSTP1, TXNRD2 in ARC and HMC lens epithelium. n = 8 independent experiments. (E) Western blot assay results revealed that the protein levels of both GSTP1 and TXNRD2 were decreased in HMC compared to ARC. mean ± SD, unpaired two-tailed Student's t-test, *P < 0.05, ****P < 0.0001.