Table 1.

Inhibitory activity of pyrrolidines 2–5 against the class II α-mannosidases GMIIb, LManII and JBMan from GH38 family enzymes.

Compound	GMIIb [IC50 (M)]	LmanII [IC50 (M)]	JBMana (%)
1a [30]	(8.8 ± 0.06) × 10−5	7.0 × 10−3	1
2a	(3.6 ± 0.20) × 10−4	n.i.a	n.i.
2b	(3.5 ± 0.17) × 10−4	n.i.a	n.i.
2c	(3.0 ± 0.18) × 10−4	n.i.a	n.i.
3a	(7.5 ± 0.35) × 10−4	n.i.a	3
3b	(9.5 ± 0.33) × 10−4	n.i.a	14
3c	(4.5 ± 0.14) × 10−4	n.i.a	39
4a	(18.0 ± 0.40) × 10−4	n.i.a	2
4b	(16.0 ± 0.40) × 10−4	n.i.a	4
4c	(19.0 ± 0.46) × 10−4	n.i.a	17
5a	(19.5 ± 0.41) × 10−4	n.i.a	21
5b	(26.5 ± 0.51) × 10−4	n.i.a	40
5c	(29.5± 0.56) × 10−4	n.i.a	38

^aInhibition in the presence of 2 mM inhibitor concentration; n.i. no inhibition.