Fig. 6.

Effect of AVV2/8-DYRK1A gene transfer on brain IkBα degradation. A) IkBα protein level, B) Ser32-phosphorylated IkBα and C) PP2A activity in brain of Cbs^+/- mice with intermediate hyperhomocysteinemia (hhcy) with (hhcy/AAV) or without (hhcy) AAV2/8-DYRK1A treatment, and control (CTL) mice. Proteins were subjected to slot blot analysis using antibodies specific to P-IkBα. After stripping, the membranes were reprobed with anti IkBα antibody. Relative protein expression was determined by normalization of the density of images from P- IkBα with that of IkBα of the same blot. Values of IkBα were obtained by normalization of images from IkBα to total proteins colored with Ponceau-S. PP2A activity was determined by immunoprecipitation phosphatase assay as described in material and methods Data were normalized to the mean of CTL mice. The results are represented as means ± SEM. n = number of mice.