Fig. 1.

Expression of OsWRKY42 is induced by either wounding or treatment with cell wall degrading enzymes but not upon treatment with Flg22. Leaves of fourteen days old rice seedlings were syringe infiltrated with any one of the following: water (control), pectinase (2 U/ml), xylanase (2 U/ml), Flg22 (100 μM). For wounding, leaves (n = 15) were punctured 8–10 times using a needle (0.45 × 13 mm). Twelve hours post treatment, leaves were harvested and processed for qRT-PCR. The relative fold change was calculated over control (for infiltration with cell wall degrading enzyme or flagellin) or untreated leaves (for wound treatment). OsGAPDH was used as endogenous control. Graph represents the mean from three biological replicates and error bar represents standard deviation