Figure 7.

Gli3 is direct downstream target of miR-218. (A) The putative binding sites of miR-218 in the 3′-UTR regions of Gli3. (B) SiHa cells were co-transfected with miR-218 mimics or NC mimics with a luc-Gli3-WT or luc-Gli3-MUT and a dual luciferase activity assay was performed. ^&P<0.05 vs. miR-218 in the 3′-UTR-MUT group. (C) Western blotting was performed to measure the expression of Gli3 protein in SiHa cells following transfection with miR-218 mimics or inhibitors. **P<0.01 vs. NC and ^##P<0.01 vs. Blank. miR, microRNA; UTR, untranslated region; NC, negative control; luc-Gli3-WT, luciferase vector containing the wild-type Gli3 3′-UTR; luc-Gli3-MUT, luciferase vector containing the mutant Gli3 3′-UTR.