Fig. 5.

Analysis of phenotype and survival rate of two transgenic chrysanthemum and WT chrysanthemum. a PCR identification results of CmDREB6 in the putative transgenic ‘Jinba’ by Hyg primers; M, 2000 Maker; 8, 9, 10, 11, 15 represents putative transgenic lines; 21 represents WT. b Relative expression level of CmDREB6 in five putative transgenic lines; The expression of CmDREB6 was determined through qRT-PCR analysis. The expression level of CmDREB6 in WT was set to 1, the error bars indicate the SE from three replicate samples. Asterisks indicate significant differences in CmDREB6 expression levels in putative transgenic lines compared with that in WT. * represents significance at p < 0.05, ** represents significance at p < 0.01. c The phenotype and survival rate of two transgenic chrysanthemum and WT plants under heat stress