Table 1.
In vitro feeding of Dermacentor reticulatus ticks and transmission of Rickettsia raoultii
| Time (h)a | Feeding unit | No. of ticks applied | Tick attachment | R. raoultii detected in bloodb | |
|---|---|---|---|---|---|
| n | % | ||||
| 24 | 1 | 10 (5m/5f) | 3 (1m/2f) | 30 (3/10) | - |
| 2 | 10 (5m/5f) | 4 (2m/2f) | 40 (4/10) | + | |
| 3 | 10 (5m/5f) | 2 (0m/2f) | 20 (2/10) | - | |
| 4 | 10 (5m/5f) | 2 (2m/0f) | 20 (2/10) | - | |
| 5 | 10 (5m/5f) | 3 (1m/2f) | 30 (3/10) | - | |
| 6 | 5 (0m/5f) | 2 (0m/2f) | 40 (2/5) | - | |
| Total | 55 | 16 | 29.1 (16/55) | ||
| 48 | 1 | 10 (5m/5f) | 5 (1m/4f) | 50 (5/10) | - |
| 2 | 10 (5m/5f) | 4 (2m/2f) | 40 (4/10) | + | |
| 3 | 10 (5m/5f) | 4 (1m/3f) | 40 (4/10) | - | |
| 4 | 10 (5m/5f) | 3 (2m/1f) | 30 (3/10) | - | |
| 5 | 10 (5m/5f) | 3 (1m/2f) | 30 (3/10) | + | |
| 6 | 5 (0m/5f) | 4 (0m/4f) | 80 (4/5) | - | |
| Total | 55 | 23 | 41.8 (23/55) | ||
| 72 | 1 | 10 (5m/5f) | 5 (1m/4f) | 50 (5/10) | + |
| 2 | 10 (5m/5f) | 4 (1m/3f) | 40 (4/10) | + | |
| 3 | 10 (5m/5f) | 4 (1m/3f) | 40 (4/10) | - | |
| 4 | 10 (5m/5f) | 2 (1m/1f) | 20 (2/10) | - | |
| 5 | 10 (5m/5f) | 4 (2m/2f) | 40 (4/10) | - | |
| 6 | 5 (0m/5f) | 4 (0m/4f) | 80 (4/5) | - | |
| Total | 55 | 23 | 41.8 (23/55) | ||
| 96 | 1 | 10 (5m/5f) | 5 (2m/3f) | 50 (5/10) | - |
| 2 | 10 (5m/5f) | 5 (1m/4f) | 50 (5/10) | - | |
| 3 | 10 (5m/5f) | 4 (1m/3f) | 40 (5/10) | - | |
| 4 | 10 (5m/5f) | 3 (2m/1f) | 30 (5/10) | + | |
| 5 | 10 (5m/5f) | 3 (1m/2f) | 30 (5/10) | + | |
| 6 | 5 (0m/5f) | 4 (0m/4f) | 80 (4/5) | + | |
| Total | 55 | 24 | 43.6 (24/55) | ||
aSampling timepoints during in vitro feeding of D. reticulatus
b+: DNA of Rickettsia raoultii detected using PCR-RLB
Abbreviations: m male, f female