Table 4.
Clinical samples tested for MDPV infection by conventional PCR and TaqMan-based PCR for MDPV detection
Samples | Region | Number of samples | Number of MDPV positive samples | Copy number for positive (copies/μL) | ||
---|---|---|---|---|---|---|
cPCR | qPCR | Both | Only | |||
Cloacal Swabs | Fujian | 15 | 2 | 4 | 7.45 × 104, 1.72× 105 | 3.59 × 101, 8.24 × 101 |
Jiangxi | 15 | 1 | 1 | 4.45 × 104 | – | |
Guangdong | 15 | 2 | 3 | 9.72× 103, 2.45 × 104 | 1.74 × 102 | |
Jiangsu | 15 | 1 | 1 | 8.61 × 104 | – | |
Zhejiang | 15 | 1 | 1 | 1.07× 104 | – | |
Embryos | Fujian | 20 | 2 | 4 | 2.89 × 103, 5.72× 103 | 7.94× 101, 1.18 × 102 |
Newly hatched Muscovy ducklings | Fujian | 20 | 4 | 5 | 3.89 × 103, 6.02× 103, 9.17× 103, 2.21× 104 | 9.47 × 101 |
cPCR means conventional PCR; qPCR means TaqMan-based real-time PCR; Both means the samples were tested both cPCR-positive and qPCR-positive; Only means the samples were tested only by qPCR-positive. “-” means no only qPCR sample available