Fig 2. S100A8/A9 expression in PASMCs of patients with PAH.
A. Immunohistochemical staining of S100A8 (green) (left figures), α-SMA (red) (center figures) and merge images (right figures) in distal pulmonary arteries of patients with IPAH (upper figures), patients with HPAH (middle figures) and without PAH (non-PAH) (lower figures). B. Immunohistochemical staining of S100A9 (green) (left figures), α-SMA (red) (center figures) and merge images (right figures) in distal pulmonary arteries of patients with IPAH (upper figures), patients with HPAH (middle figures) and patients without PAH (non-PAH) (lower figures). C. Immunocytochemical analysis of S100A8 (green) (left figures), S100A9 (green) (center figures) and negative control staining without 1st antibody (left figures) in cultured PASMCs of patients with IPAH (upper figures), patients with HPAH (middle figures) and patients without PAH (non-PAH) (lower figures). Nuclear staining was performed by use of DAPI (blue). Bar = 100 μm.