Fig 8. AFM-IR images of the four spectroscopic parameters acquired on the same area of a cancellous bone sample.

Maps were derived from images acquired at six wavenumbers: 1128 cm^-1, 1096 cm^-1, 1030 cm^-1, 1020 cm^-1 (mineral band), 1690 cm^-1, and 1660 cm^-1 (amide). Those maps, particularly the mineral-to-matrix ratio and the collagen crosslink ratio, show layers of high and low intensities. Color scales are the AFM-IR intensity ratios.