Skip to main content
. 2018 Sep 4;13(9):e0203397. doi: 10.1371/journal.pone.0203397

Fig 5. VRK1-overexpression leads to the formation of enlarged and misshapen acini in 3D matrigel culture.

Fig 5

(A) Representative brightfield images of 3D acini formed by control, 3XF-VRK1- overexpressing cells and 3XF- VRK1D177A-overexpressing MCF10a cells at days 4, 5.5, 7, and 12 of 3D culture. Scale bar = 100μm. (B) Quantification of the acinus areas at days 4, 5.5, 7, and 12 of 3D culture [n = ~70, vector (blue circles) or 3XF-VRK1 (green circles); n = ~35, 3XF-VRK1D177A (red circles)]. Bars indicate the median. (Mann-Whitney test, ***p<0.001). (C and D) Confocal immunofluorescence analysis of 3D acini formed by control and 3XF-VRK1 overexpressing MCF10a cells after 7 days of culture. Acini were stained for the epithelial cell-cell junction marker E-cadherin (C) and basal polarity marker laminin V (D). Scale bar = 100μm.