Fig 2. Total reactive oxygen species production.

S. Typhimurium strains were incubated at a MOI of 100 with each cell type and ROS were evaluated at 1 and 3 hpi. The amount of ROS was determined by quantifying DCF fluorescence in neutrophils and macrophages co-cultured with the parental S. Typhimurium 14028s, and the ΔarcA and ΔarcB mutants. Values indicate Arbitrary Fluoresce Units (AFU) normalized by the number of bacteria recovered. One-way ANOVA followed by Bonferroni post hoc test.; no significant differences found at α = 0.05. Biological replicates n = 5, three technical replicates per experiment.