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. 2018 Aug 22;14(8):e1007249. doi: 10.1371/journal.ppat.1007249

Fig 2. Granular localization of 7-Helix-1 in female P. falciparum gametocytes.

Fig 2

(A) Immunolocalization of 7-Helix-1 in the sexual stages. Gametocytes (GC stage II–V) and activated gametocytes (aGC; at 2, 10 and 15 min p.a.) of WT NF54 were immunolabeled with mouse anti-7-Helix-1rp1 antisera (green) and counterlabeled with rabbit antibodies directed against Pfs230 and Pfs25 as indicated (red). (B) Female-specific expression of 7-Helix-1. WT NF54 gametocytes were immunolabeled with mouse anti-7-Helix-1rp1 antisera (green) and counterlabeled with anti-Pfs25 antisera (red). Nuclei (in A and B) were highlighted by Hoechst33342 nuclear stain (blue). Bar, 5 μm. Results are representative of five independent experiments.