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. 2018 Aug 22;14(8):e1007266. doi: 10.1371/journal.ppat.1007266

Fig 4. NLRC3 deficiency promotes differentiation of CD4+ T and IL-2 production in vivo.

Fig 4

Naïve CD4+ T cells from CD45.1+ WT and CD45.2+ Nlrc3-/- mice were mixed at a 1:1 ratio and competitively transferred via tail vein injection into Rag2-/- recipient mice. Then recipient mice were infected with M. tuberculosis and were harvested at 3w.p.i.. (A) Lung cells were restimulated with M. tuberculosis lysate directly ex vivo and the intracellular production of IFN-γ by CD4+ T cells was determined. Representative FACs plots depicting gating of CD4+ T cells are shown. Gating strategies to evaluate cytokine production by WT and Nlrc3-/- CD4+ T cells are provided. Numbers in the quadrants indicate the percent cells in each. Pooled data are presented in the right panel. (B) Expression of CD69 by lung CD4+ T cells. (C) Expression of IL-2 by lung CD4+ T cells. Pooled data are presented in the right panel. Data shown in (A, C) are the mean ±SD. *P < 0.05 and **P < 0.01. Data are representative of three independent experiments with similar results.