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Schematic of PTE workflow. Monodispersed polyacrylamide (PAA) beads are added to the PCR reaction mix (a) and soaked with enzymes and molecules (b). After removing excess aqueous phase (c), oil with stabilizing surfactant is introduced (d) and the mixture is uniformly emulsified by vortexing (e). For digital droplet PCR, target sequences are encapsulated and amplified in the droplets, generating a detectable fluorescence signal (f).
