(A, B) Dantrolene decreases GLuc-SERCaMP and endogenous SERCaMP release during
ad lib access to high fat pellets. Rats were administered
dantrolene (15mg/kg) or vehicle for 7 days during diet (mean ± SEM,
n=6 rats/group, *p<0.05, 2-way ANOVA, Sidak’s
multiple comparison test). (C) Decreased food consumption among dantrolene
injected rats (mean ± SEM, n=6 rats/group,
****p<0.0001, 2-way ANOVA). (D) Dantrolene
decreases BiP mRNA levels in liver tissue (data expressed as fold change
(2^-ΔΔCt) relative to vehicle-treated rats (mean ± upper
and lower limits (2ΔΔCt±SD), n=6
rats/group, multiple t-test, Holm-Sidak method (dantrolene versus vehicle)
transformed from ΔCt±SD, *p=0.01).